Wide-field functional imaging of blood flow and hemoglobin oxygen saturation in the rodent dorsal window chamber

► Present detailed protocol to surgically implant dorsal window chamber in mice and hamsters. ► Present detailed protocol to perform laser speckle imaging on dorsal window chamber. ► Present detailed protocol to perform multispectral oxygenation imaging on dorsal window chamber. ► Wide-field optical imaging complements intravital microscopy of dorsal window chamber.