Opposite effects of ANP receptors in attenuation of LPS-induced endothelial permeability and lung injury

► This study examined the role of ANP and its receptors to the modulation of LPS-induced lung injury. ► Inhibition of NPR-A augmented LPS-induced EC permeability and blocked protective effects of exogenous ANP. ► Inhibition of ANP clearance receptor NPR-C attenuated LPS-induced barrier disruptive effects. ► In vivo NPR-A inhibitor exacerbated LPS-induced lung injury, whereas inhibition of NPR-C suppressed LPS-induced lung injury.