A novel and simple method for culturing pericytes from mouse brain

► We describe a novel simple method of culturing brain pericytes. ► Cultures are highly homogeneous, expressing NG2, PDGFβ-receptor, and CD146. ► Closely align with endothelial vascular cords in matrigel co-culture. ► Can be passaged to high number without premature differentiation. ► Both low and high passage cells upregulate α-SMA when cultured in DMEM + 10% FBS.