Akt inactivation induces endoplasmic reticulum stress-independent autophagy in fibroblasts from patients with Pompe disease

► Induction of ER stress-independent autophagy was observed in Pompe fibroblasts. ► Inactivation of Akt was observed in patient fibroblasts lacking ER stress. ► Activation of Akt suppressed the induction of autophagy in patient fibroblasts. ► Insulin improved the localization of rhGAA with lysosomes in patient fibroblasts.