Applying and testing the conveniently optimized enzyme mismatch cleavage method to clinical DNA diagnosis

► CHIPS demonstrated very high utility for mutation screening of clinical genetics. ► CHIPS can apply to autosomal dominant, recessive and X-linked inheritance diseases. ► CHIPS detected all DNA variations with 100% sensitivity and specificity. ► This low-technology system is comparable to recent new-technology machines. ► CHIPS is ready to use and potentially disseminate to any laboratories in the world.