Examination of bacterial resistance to exogenous nitric oxide

► We evaluate the ability of bacteria to develop a resistance to exogenous NO. ► Nitrosothiol nanoparticles were utilized as an exogenous antimicrobial model. ► Prolonged exposure to sub-therapeutic NO did not result in increased resistance. ► Single-dose exposure to bactericidal NO did not result in increased resistance. ► These assays may serve as a blueprint for future NO resistance studies.