The three isoforms of nitric oxide synthase distinctively affect mouse nocifensive behavior

► We examined nocifensive behavior in nitric oxide synthases genetically deficient mice. ► We measured current vocalization threshold for specific nerve sensory fibers in mice. ► Each nitric oxide synthase distinctively affects baseline mouse nocifensive behavior. ► NOS1 affects NOS2, NOS3 and chemokine expression in cortex and spinal cord.