Larazotide acetate regulates epithelial tight junctions in vitro and in vivo

► Larazotide acetate (LA) inhibits gliadin-induced actin and ZO-1 redistribution. ► LA inhibits cytokine-induced permeability. ► LA inhibits gliadin translocation across Caco-2 cell monolayers. ► LA preserves tight junction structure and function in gluten-sensitive mice. ► LA is a novel compound with therapeutic value in celiac disease management.