Effects of the anandamide uptake blocker AM404 on food intake depend on feeding status and route of administration

► Examination of the anandamide uptake blocker AM404 on feeding in fast and satiation. ► AM404 (i.p.) is anorectic in partially satiated rats via PPARα-independent mechanisms. ► AM404 (i.c.v.) induces appetite in fasted rats, and this effect is abolished by SR141716A (i.p.). ► We show the multimodal action of the endocannabinoid signaling on feeding regulation.