Characterization of brassinosteroid-regulated proteins in a nuclear-enriched fraction of Arabidopsis suspension-cultured cells

► We examine brassinosteroid (BR) function in Arabidopsis cells. ► Nuclear-targeted proteomics was applied to cells with BR added or inhibited. ► BR-regulated proteins were characterized by 2D-PAGE and nano-LC-MS/MS. ► Some identified proteins may be involved in BR-responsive chromatin remodeling.