کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10840283 | 1067621 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Physiological implications of trehalase from Phaseolus vulgaris root nodules: partial purification and characterization
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کلمات کلیدی
SDSPhaseolus vulgarisDASPAGE2-(N-morpholino)-ethanesulfonic acid - 2- (N-مورفولینو) -اتان سولفونیک اسیدEDTA - اتیلن دی آمین تترا استیک اسید ethylenediamine tetraacetic acid - اسید اتیلنیدین تتراستیکpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدTris–HCl - تریس-HClTrehalase - تهرالازdays after sowing - روز بعد از کاشتsodium dodecyl sulfate - سدیم دودسیل سولفاتCarbon metabolism - متابولیسم کربنMeS - مسNodules - نودلdry weight - وزن خشک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Physiological implications of trehalase from Phaseolus vulgaris root nodules: partial purification and characterization Physiological implications of trehalase from Phaseolus vulgaris root nodules: partial purification and characterization](/preview/png/10840283.png)
چکیده انگلیسی
The purification and characterization of trehalase from common bean nodules as well as the role of this enzyme on growth, nodulation nitrogen fixation by examining the effects of the trehalase inhibitor validamycin A, was studied. Validamycin A did not affect plant and nodule mass, neither root trehalase and nitrogenase activity; however this treatment applied at the time of sowing increased nodule number about 16% and decreased nodule trehalase activity (16-fold) and the size of nodules. These results suggest that nodule trehalase activity of Phaseolus vulgaris could be involved in nodule formation and development. In addition, acid trehalase (EC 3.2.1.28) was purified from root nodules by fractionating ammonium sulfate, column chromatography on DEAE-sepharose and sephacryl S-300, and finally on native polyacrylamide gel electrophoresis. The purified homogeneous preparation of native acid trehalase exhibited a molecular mass of 42 and 45 kDa on SDS-PAGE. The enzyme has the optimum pH 3.9, Km of 0.109 mM, Vmax of 3630 nkat mg-1 protein and is relatively heat stable. Besides trehalose, it shows maximal activity with sucrose and maltose and, to a lesser degree melibiose, cellobiose and raffinose, and it does not hydrolyze on lactose and turanose. Acid trehalase was activated by Na+, Mn2+, Mg2+, Li+, Co2+, K+ and inhibited by Fe3+, Hg+ and EDTA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 4, April 2005, Pages 355-361
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 4, April 2005, Pages 355-361
نویسندگان
Noel A. Tejera GarcÃa, Carmen Iribarne, Miguel López, José A. Herrera-Cervera, Carmen Lluch,