کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10840439 | 1067692 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tilia platyphyllos Scop.-Tuber brumale Vittad. vs. T. platyphyllos Scop.-T. borchii Vittad. ectomycorrhizal systems: a comparison of structural and functional traits
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کلمات کلیدی
PVPPα-naphthylacetic acidGOGATGDHBAPDTESDSPVPNaAPDA1,4-dithiothreitol - 1،4-dithiothreitolBSA - BSAInternal transcribed spacer (ITS) - Sparker داخلی رونویسی شده (ITS)Albumin from bovine serum - آلبومین از سرم گاوEDTA - اتیلن دی آمین تترا استیک اسید ergosterol - ارگوسترولsodium dodecyl sulphate - سدیم دودسیل سولفاتpotato dextrose agar - سیب زمینی دگزستروز آگارpolyvinylpyrrolidone - پلی وینیل پیرولیدونpolyvinylpolypyrrolidone - پی ویوی پلیپیرلیدونCHAPS - چاپسglutamate dehydrogenase - گلوتامات دهیدروژنازglutamate synthase - گلوتامات سنتازGlutamine synthetase - گلوتامین سنتتاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ectomycorrhizae are mutualistic associations of several species of fungi with higher plants. Their formation involves alterations in the morphology and cell structure of the plant root and fungal mycelium. These modifications are correlated with mRNA and protein synthesis in the two symbionts. To gain more information about structural and functional traits involved in ectomycorrhizal formation, two “in vitro” ectomycorrhizal systems, set up by the inoculation of Tilia platyphyllos Scop. roots with either Tuber brumale Vittad. or T. borchii Vittad. mycelia, were investigated. Different parameters such as, fungal volume ratio, fungal biomass, plant and fungal transcript levels, specific enzymes activity and protein patterns were evaluated. In T. platyphyllos-T. brumale ectomycorrhizal tissue all the molecular and morphometrical approaches revealed a higher fungal biomass, volume and transcript as well as higher fungal protein levels respect to the host plant, suggesting that the fungal genes and proteins are up regulated after the establishment of symbiosis. These results are completely divergent from that obtained in T. platyphyllos Scop.-T. borchii Vittad. ectomycorrhizal system, leading us to hypothesise a different role of the fungal partner in the mycorrhization process according to the species it belongs to.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 7, July 2005, Pages 709-716
Journal: Plant Physiology and Biochemistry - Volume 43, Issue 7, July 2005, Pages 709-716
نویسندگان
Sabrina Zeppa, Davide Sisti, Raffaella Pierleoni, Lucia Potenza, Michele Guescini, Luciana Vallorani, Vilberto Stocchi,