کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10840798 | 1067854 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reduced expression of a protein homologous to glycogenin leads to reduction of starch content in Arabidopsis leaves
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We used the yeast and mammalian glycogenin sequences to identify homologous sequences in Arabidopsis. Database searches revealed at least eight genes with varying degrees of homology to the yeast and mammalian sequences. However, only one of these was predicted to contain a transit peptide for localisation to the chloroplast, the site of starch synthesis. We have called this gene plant glycogenin-like starch initiation protein 1 (PGSIP1) and we show that it exists as a member of a gene family, probably comprising six members. Knockout of PGSIP1 expression in Arabidopsis results in reduction of the starch content in leaves. This demonstrates its crucial role in starch biosynthesis. Identification of homologous genes in rice, wheat, maize, potato and barley shows that PGSIP1-type genes are of widespread occurrence. The phenotype of PGSIP1 knockout lines and homology of the deduced PGSIP1 protein sequence with glycogenin proteins suggests that this protein is involved in starch biosynthesis and that it may have a starch-priming function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 168, Issue 2, February 2005, Pages 501-509
Journal: Plant Science - Volume 168, Issue 2, February 2005, Pages 501-509
نویسندگان
Manash Chatterjee, Pierre Berbezy, Darshna Vyas, Steve Coates, Tina Barsby,