کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10841078 | 1067879 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization and expression of chalcone synthase gene from Ginkgo biloba
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
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چکیده انگلیسی
The genomic DNA sequence of chalcone synthase (CHS) gene was cloned from Ginkgo biloba. The Gbchs was 1295Â bp long and composed of two exons and one intron, one of the typical features of chalcone synthase genes. The genomic Southern blot analysis indicated that Gbchs belonged to a multigene family. RT-PCR analyses revealed that Gbchs expressed differentially in the root, stem and leaf tissues of G. biloba, and the expression was induced by UV-B and wounding treatments. The recombinant GbCHS protein was successfully expressed in Escherichia coli strain M15 [pREP4] with pQE30 vector and the result showed that the expressed GbCHS protein had molecular weight of about 42Â kDa, a size matching that of the predicted by bioinformatic analysis. This study provides useful information for further studying this gene and its function in ginkgo flavonoids biosynthetic pathway in G. biloba, the so-called “living fossil” plant possessing many pharmaceutical properties for human health.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 168, Issue 6, June 2005, Pages 1525-1531
Journal: Plant Science - Volume 168, Issue 6, June 2005, Pages 1525-1531
نویسندگان
Yongzhen Pang, Guoan Shen, Weisheng Wu, Xuefen Liu, Juan Lin, Feng Tan, Xiaofen Sun, Kexuan Tang,