کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10841086 | 1067879 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of osmotic stress on the activity of H+-ATPase and the levels of covalently and noncovalently conjugated polyamines in plasma membrane preparation from wheat seedling roots
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کلمات کلیدی
O-PhenLRWCSPDMGBSPMOsmotic stress - استرس اسمزیSpermine - اسپرمSpermidine - اسپرمیدینPlasma membrane H+-ATPase - غشاء پلاسما H + -ATPasePlasma membrane - غشای پلاسماPUT - قرار دادنputrescine - قرار دادنLeaf relative water content - محتوای نسبی آب برگPAS - نهPolyamines - پلی آمینpolyethylene glycol - پلی اتیلن گلیکولPEG - پلیاتیلن گلیکول
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of osmotic stress on the activity of H+-ATPase and the levels of covalently and noncovalently conjugated polyamines in plasma membrane preparation from wheat seedling roots Effect of osmotic stress on the activity of H+-ATPase and the levels of covalently and noncovalently conjugated polyamines in plasma membrane preparation from wheat seedling roots](/preview/png/10841086.png)
چکیده انگلیسی
The influence of osmotic stress on the H+-ATPase (EC 3.6.1.35) activity and the contents of covalently and noncovalently conjugated polyamines were investigated using plasma membrane (PM) vesicles isolated from the roots of wheat (Triticum aestivum L.) seedlings with different drought-tolerance by the sucrose gradient centrifugation method. Results showed that after polyethylene glycol (PEG) 6000 (â0.55 MPa) treatment for 7 days, leaf relative water content (LRWC), relative dry weight increase rate (RDWIR) of seedlings of drought-sensitive Yangmai No. 9 cv. decreased more markedly than that of drought-tolerant Yumai No. 18 cv., while plasma membrane (PM) H+-ATPase activity, the content ratio of noncovalently conjugated polyamine (NCC-PAs) NCC-Spd + NCC-Spm to NCC-Put and the sum contents of covalently conjugated polyamines (CC-PAs) CC-Put + CC-Spd of PM of Yumai No. 18 cv. increased more markedly than that of Yangmai No. 9 cv. Exogenous Spd treatment alleviated osmotic stress injury to Yangmai No.9 seedlings, coupled with marked increases of NCC-Spd and NCC-Spm levels and H+-ATPase activity in the root PM vesicle. Under PEG osmotic stress, the concomitant treatment of drought-tolerant cv. Yumai No. 18 seedlings with methylglyoxyl-bis (guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC), and phenanthrolin (o-Phen), an inhibitor of transglutaminase (TGase), caused a decrease of the NCC-Spd + NCC-Spm: NCC-Put ratio and a reduction of sum contents of CC-Put + CC-Spd, respectively. This was associated with decreased PM-H+-ATPase activity and tolerance of seedlings to osmotic stress. Together, these results suggest that tolerance of the wheat seedlings to osmotic stress is associated with the activity of H+-ATPase and the contents of NCC-Spd and NCC-Spm together with CC-Put and CC-Spd in the plasma membrane of roots.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 168, Issue 6, June 2005, Pages 1599-1607
Journal: Plant Science - Volume 168, Issue 6, June 2005, Pages 1599-1607
نویسندگان
H.P. Liu, B.J. Yu, W.H. Zhang, Y.L. Liu,