High-level over-expression, purification, and crystallization of a novel phospholipase C/sphingomyelinase from Pseudomonas aeruginosa

► Overexpression of the secreted phospholipase C/sphingomyelinase PlcHR2 from P. aeruginosa in native and Se-methionine substituted form. ► Purification and crystallisation of PlcHR2. ► Crystalographic analysis and data collection of PlcHR2 micro-crystals.