Expression and purification of chimeric peptide comprising EGFR B-cell epitope and measles virus fusion protein T-cell epitope in Escherichia coli

► A first reported antigen peptide based on EGFR237-267 for targeting the dimer interface of hEGFR. ► A first reported chimeric peptide comprising B-cell epitope from EGFR and promiscuous T-cell epitope MVF. ► A first reported recombinant chimeric peptide. ► A strategy for preparing easily-degraded small peptide with E. coli expression system.