Production and binding analyses of a humanised scFv against a cryptic epitope on tumour-associated fibronectin

► A single-chain Fv can be successful constructed from a humanized BC-1 immunoglobulin. ► The scFv (HuBC-1) is more stable the its murine counterpart. ► The HuBC-1 scFv retains full binding function by ELISA, BIACore analyses and flow cytometry. ► The HuBC-1 scFv is stable and localizes to tumours in an animal model.