Heterologous expression and characterization of soluble recombinant 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from Actinosynnema pretiosum ssp. auranticum ATCC31565 through co-expression with Chaperones in Escherichia coli

► We obtain soluble recombinant Ap-DAHPS via coexpression with chaperones in Escherichia coli. ► DAHPS from Actinosynnema pretiosum has been characterized. ►Ap-DAHPS activity is inhibited by high concentration of phosphoenolpyruvate. ► Mn2+ and Co2+ significantly increase the activity of Ap-DAHPS.