Expression and purification of soluble porcine CTLA-4 in yeast Pichia pastoris

► Soluble porcine CTLA-4 was successfully expressed and purified in Pichia pastoris system. ► Removing N-glycosylation sites improved the soluble porcine CTLA-4 expression and purification. ► Soluble porcine CTLA-4 can bind to a porcine CD80-expressing B-cell lymphoma cell line.