Cultivation at 6-10 °C is an effective strategy to overcome the insolubility of recombinant proteins in Escherichia coli

► Escherichia coli was cultivated at 6-10 °C to produce recombinant proteins. ► E. coli strains without cold adapted chaperones grew at the extremely low temperatures. ► The active soluble proteins were produced in large quantity under this condition. ► This strategy is effective for overcoming the insolubility of recombinant proteins.