Thermophilic Thermotoga maritima ribose-5-phosphate isomerase RpiB: Optimized heat treatment purification and basic characterization

► Recombinant Thermotoga maritima ribose-5-phosphate isomerase RpiB was over-produced in Escherichia coli. ► High-purity thermostable enzyme was obtained through optimized heat treatment. ► Thermostable ribose-5-phosphate isomerase RpiB had a total turn-over number (TTN) of >200,000,000.