Recombinant expression, purification, and characterization of scorpion toxin BmαTX14

► Toxin rBmαTX14 was produced by the recombinant expression in Escherichia coli. ► BmαTX14's structure is stable with or without His6-tag. ► BmαTX14 selectively inhibits the fast inactivation of mNav1.4 channel. ► The activity of BmαTX14 inhibiting channel inactivation is less affected by His6-tag.