Synthesis and purification of a toxin-linked conjugate targeting epidermal growth factor receptor in Escherichia coli

► We fused LP1 to an anti-EGFR scFv. ► We expressed EGFR/LP1 in Escherichia coli. ► We identified the GBM-specific cytotoxicity of EGFR/LP1. ► We determined the antitumor effects of EGFR/LP1 toward GBM.