Expression and purification of non-N-glycosylated porcine interleukin 3 in yeast Pichia pastoris

► The codon-optimized non-N-glycosylated porcine IL3 was expressed and purified in Pichia pastoris. ► The non-N-glycosylated porcine IL3 still keeps the original stem cell mobilization function. ► The high-mannose N-glycosylation problem of P. pastoris expressed porcine IL3 was resolved.