Purification and characterization of riproximin from Ximenia americana fruit kernels

► The type II RIP riproximin was purified from the fruit kernels of Ximenia americana. ► The newly established purification procedure is described. ► Purified batches showed reproducible biochemical and biological properties. ► Purified fruit kernel riproximin consisted of a mixture of closely related isoforms. ► The purified riproximin provides a reliable basis for its further development.