Cloning, expression and characterization of the recombinant Yersinia pseudotuberculosisl-asparaginase

► We created Escherichiacoli strain with Yersinia pseudotuberculosisl-asparaginase expression. ► The enzyme has similar biochemical properties with E. coli type II l-asparaginase. ► Y. pseudotuberculosisl-asparaginase has low activity towards l-glutamine. ► Received l-asparaginase inhibits growth of tumor cells both in vitro and in vivo.