Efficient expression of recombinant soluble human FcγRI in mammalian cells and its characterization

► Efficient expression of recombinant soluble human FcγRI (rshFcγRI) in CHO cells. ► Stable CHO clones were established by using gene-amplification system. ► Established clones produced rshFcγRI under high-density continuous culture. ► The binding affinities and structure of purified rshFcγRI were determined. ► These results might be valuable for further studies of human FcγRI.