Cloning, soluble expression, rapid purification and characterization of human Cofilin1

► Human Cofilin1 protein was successfully expressed as soluble form in Escherichia coli BL21. ► The optimized induction conditions for Cofilin1 expression were identified. ► Cofilin1 was rapidly and efficiently purified by one-step Ni-NTA affinity chromatography. ► Purified Cofilin1 protein possessed good bioactivity. ► Purified Cofilin1 obtained preserved high immunogenicity.