Expression and purification of recombinant human Mig in Escherichia coli and its comparison with murine Mig

► We expressed and purified recombinant human CXCL9/Mig with an Escherichia coli expression system. ► The process is easy, fast and cost effective. ► The product is of high purity and low level of endotoxin. ► The product is of high bio-activity in vivo and in vitro. ► We compared the functions of human Mig in comparison with murine Mig in the mouse model.