Expression of active recombinant human gastric lipase in Nicotiana benthamiana using the CPMV-HT transient expression system

► Successful transient expression in plants of active human gastric lipase (hGL). ► Development of an effective method for the extraction of active hGL from plants. ► Development of methods for purifying active hGL from plant extracts. ► Demonstration that plant-expressed hGL retains the properties of the native enzyme.