Production and single-step purification of EGFP and a biotinylated version of the Human Rhinovirus 14 3C protease

► Cloning, growth and single step affinity purification of soluble EGFP. ► Cloning, growth and single step purification of a biotinylated variant of Human Rhinovirus 14 3C protease. ► Purified enzymes produced in yields greater than previously described and comparable to commercially available sources.