کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10843374 | 1069231 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ribosomal synthesis and in situ isolation of peptide molecules in a cell-free translation system
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Ribosomal synthesis and in situ isolation of peptide molecules in a cell-free translation system Ribosomal synthesis and in situ isolation of peptide molecules in a cell-free translation system](/preview/png/10843374.png)
چکیده انگلیسی
Although the cell-free translation system is now widely accepted as an efficient platform for production, engineering and screening of recombinant proteins, it has not been successfully used for the synthesis of peptide molecules mainly due to low expression yields and rapid proteolysis of the expressed peptides. In this study, we propose a novel strategy for rapid expression and recovery of peptide molecules which involves the rational design of template DNA and heterogenous cell-free translation reaction in the presence of affinity beads. Various peptide molecules which were not expressed in a detectable level were successfully expressed and recovered in situ in a substantial yield. We expect that the presented approach will be widely used as a versatile platform for the generation of a variety of peptide molecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 71, Issue 1, May 2010, Pages 16-20
Journal: Protein Expression and Purification - Volume 71, Issue 1, May 2010, Pages 16-20
نویسندگان
Kyung-Ho Lee, Yong-Chan Kwon, Sung Joon Yoo, Dong-Myung Kim,