کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10843391 | 1069231 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and purification of recombinant human inward rectifier K+ (KCNJ) channels in Saccharomyces cerevisiae
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression and purification of recombinant human inward rectifier K+ (KCNJ) channels in Saccharomyces cerevisiae Expression and purification of recombinant human inward rectifier K+ (KCNJ) channels in Saccharomyces cerevisiae](/preview/png/10843391.png)
چکیده انگلیسی
The inward rectifier family of potassium (KCNJ) channels regulate vital cellular processes including cell volume, electrical excitability, and insulin secretion. Dysfunction of different isoforms have been linked to numerous diseases including Bartter's, Andersen-Tawil, Smith-Magenis Syndromes, Type II diabetes mellitus, and epilepsy, making them important targets for therapeutic intervention. Using a family-based approach, we succeeded in expressing 10 of 11 human KCNJ channels tested in Saccharomyces cerevisiae. GFP-fusion proteins showed that these channels traffic correctly to the plasma-membrane suggesting that the protein is functional. A 2-step purification process can be used to purify the KCNJ channels to >95% purity in a mono-dispersed form. After incorporation into liposomes, 86Rb+ flux assays confirm the functionality of the purified proteins as inward rectifier potassium channels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 71, Issue 1, May 2010, Pages 115-121
Journal: Protein Expression and Purification - Volume 71, Issue 1, May 2010, Pages 115-121
نویسندگان
Nazzareno D'Avanzo, Wayland W.L. Cheng, Xiaobing Xia, Liang Dong, Pavel Savitsky, Colin G. Nichols, Declan A. Doyle,