کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10843731 | 1069291 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Efficient production, purification, and application of egg yolk antibodies against human HLA-A*0201 heavy chain and light chain (β2m)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The importance of eggs as a source of specific antibodies is well recognized. Egg yolk contains 8-20Â mg immunoglobulins (IgY) per milliliter. However, the major problem in separating IgY is to remove the high concentrations of lipids in egg yolk. We first used water dilution method to get the supernatant containing IgY, then purified the antibody by caprylic acid-ammonium sulfate method, and obtained specific antibody with satisfactory purity and activity. By comparison of these several methods, each has its advantages, one can be chosen to purify IgY according to practical need. The purified IgY produced by the immunized chickens can stain the human peripheral blood mononuclear cell effectively when labeled with fluorescent FITC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 44, Issue 1, November 2005, Pages 45-51
Journal: Protein Expression and Purification - Volume 44, Issue 1, November 2005, Pages 45-51
نویسندگان
Guang-Ping Ruan, Li Ma, Xiao-Wei He, Min-Jie Meng, Yong Zhu, Ming-Qian Zhou, Zhi-Ming Hu, Xiao-Ning Wang,