کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10843910 | 1069296 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Process intensification of fluidized bed dye-ligand adsorption of G3PDH from unclarified disrupted yeast: A case study of the performance of a high-density steel-agarose pellicular adsorbent
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The development of a process intensified primary capture step for the direct selective recovery of intracellular proteins from very dense particulate-containing yeast extract has been explored. The purification of glyceraldehyde 3-phosphate dehydrogenase from bakers' yeast was chosen as a potential demonstration of this approach. A high throughput (50%, w/v, yeast extracts at a superficial linear velocity of 450 cm hâ1) was achieved by adoption of a high-density adsorbent (UpFront steel-agarose; Ï = 2.65 g mlâ1) derivatized with selective ligand chemistries (Cibacron Blue 3GA). This should ultimately minimize adsorption time and maximize process efficiency of fluidized bed adsorption.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 42, Issue 1, July 2005, Pages 160-165
Journal: Protein Expression and Purification - Volume 42, Issue 1, July 2005, Pages 160-165
نویسندگان
Tau Chuan Ling, Andrew Lyddiatt,