کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10870650 1074017 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Delineation of the TRAK binding regions of the kinesin-1 motor proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Delineation of the TRAK binding regions of the kinesin-1 motor proteins
چکیده انگلیسی
Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 cargo domain, rationally designed C-terminal truncations of KIF5A and KIF5C were generated and their co-association with TRAK1/2 determined by quantitative co-immunoprecipitations following co-expression in mammalian cells. Three contributory regions forming the TRAK2 binding site within KIF5A and KIF5C cargo binding domains were delineated. Differences were found between TRAK1/2 with respect to association with KIF5A.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 587, Issue 23, 29 November 2013, Pages 3763-3769
نویسندگان
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