کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10870661 1074017 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Site-directed mutagenesis of a fatty acid elongase ELO-like condensing enzyme
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Site-directed mutagenesis of a fatty acid elongase ELO-like condensing enzyme
چکیده انگلیسی
The condensation step of fatty acid elongation is the addition of a C2 unit from malonyl-CoA to an acyl primer catalyzed by one of two families of enzymes, the 3-ketoacyl-CoA synthases and the ELO-like condensing enzymes. 3-Ketoacyl-CoA synthases use a Claisen-like reaction mechanism while the mechanism of the ELO-catalyzed condensation reaction is unknown. We have used site-directed mutagenesis of Dictyostelium discoideum EloA to identify residues important to catalytic activity and/or structure. Mutation of highly conserved polar residues to alanine resulted in an inactive enzyme strongly suggesting that these residues play a role in the condensation reaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 587, Issue 23, 29 November 2013, Pages 3837-3842
نویسندگان
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