Can we trap the S3YZ metalloradical intermediate during the S-state transitions of Photosystem II? An EPR investigation

We report the trapping of two metalloradical intermediates corresponding to the transitions S2 to S3 and S3 to S0 of the oxygen evolving complex (OEC) of Photosystem II (PSII), in preparations containing methanol, at temperatures near that of half inhibition of the respective S-state transitions. The first intermediate, with an EPR width of 160 G, is assigned to S2YZ, based on its similarity to the one previously characterized after trapping at 10 K. The second with a splitting of ∼80 G is tentatively assigned to S3YZ. The S3YZ EPR signal is weaker than the S2YZ one, and both are stable at cryogenic temperatures.