Regulatory mechanism of PAC1 gene expression via Sp1 by nerve growth factor in PC12 cells

► Nerve growth factor induces PAC1 gene expression in PC12 cells. ► SP1 site at −282/−273 has a key role for augmentation of PAC1 gene expression induced by NGF. ► U0126, an MEK inhibitor, or Mithramycin A, an SP1 inhibitor, attenuates the promoter activity of PAC1 gene in PC12 cells.