Biochemical characterization of the very long-chain fatty acid elongase ELOVL7

► Progression of the VLCFA elongation cycle enhances ELOVL7 activity. ► ELOVL7 activity can be measured by reconstituting into proteoliposomes. ► Purified ELOVL7 exhibited high activity toward C18-CoA. ► Purified ELOVL7 exhibited Km values for its substrates in the μM order.