The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P

► The recombinant CYP701B1 protein was produced in Pichia yeast. ► The CYP701B1 was identified as Physcomitrella patensent-kaurene oxidase. ► The enzyme activity showed resistance to a P450 inhibitor uniconazole-P. ► This resistant property was not found in KOs of vascular plants such as Arabidopsis.