Filter centrifugation as a sampling method for miniaturization of extracellular fungal enzyme activity measurements in solid media

► A novel sampling method to determine extracellular fungal enzyme activities in agar. ► Centrifugation recovers liquid phase from sample including extracellular enzymes. ► No addition of water or buffer necessary and thus no dilution of the sample. ► Application for screening approaches, functional enzyme fingerprints and investigation of microcosms.