کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10876919 | 1075787 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heat shock protein 70 is required for tabtoxinine-β-lactam-induced cell death in Nicotiana benthamiana
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کلمات کلیدی
RT-PCRqRT-PCRHspPCDMSXl-methionine sulfoximineProgrammed cell death - مرگ برنامهریزی شده یاختهreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسpolymerase chain reaction - واکنش زنجیره ای پلیمرازquantitative real time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPCR - واکنش زنجیرهٔ پلیمرازHypersensitive response - پاسخ حساسیتHeat shock protein - پروتئین شوک حرارت
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Heat shock protein 70 is required for tabtoxinine-β-lactam-induced cell death in Nicotiana benthamiana Heat shock protein 70 is required for tabtoxinine-β-lactam-induced cell death in Nicotiana benthamiana](/preview/png/10876919.png)
چکیده انگلیسی
Tabtoxinine-β-lactam (TβL), a non-specific bacterial toxin, is produced by Pseudomonas syringae pv. tabaci, the causal agent of tobacco wildfire disease. TβL causes death of plant cells through the inhibition of glutamine synthetase, which leads to an abnormal accumulation of ammonium ions and the characteristic necrotic wildfire lesions. To better understand the mechanisms involved in TβL-induced cell death, we studied its regulation in Nicotiana benthamiana. TβL-induced lesions, similar to those in controls, could be observed in SGT1-, RAR1- and Hsp90-silenced plants. In contrast, Hsp70-silenced plants showed suppression of lesion formation. Expression of hin1, a marker gene for the hypersensitive response (HR), which is a characteristic of programmed cell death in plants, was strongly induced in controls by TβL treatment but only slightly in Hsp70-silenced plants. However, in these TβL-treated Hsp70-silenced plants, the amount of ammonium ions was considerably increased. Furthermore, the silencing of Hsp70 also suppressed l-methionine sulfoximine-induced cell death and hin1 expression and caused the over-accumulation of ammonium ions. When inoculated directly with P. syringae pv. tabaci, Hsp70-silenced plants showed only reduced symptoms. Our results suggest that the TβL-induced pathway to cell death in N. benthamiana is at least partially similar to HR response, and that Hsp70 might play an essential role in these events.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 171, Issue 2, 15 January 2014, Pages 173-178
Journal: Journal of Plant Physiology - Volume 171, Issue 2, 15 January 2014, Pages 173-178
نویسندگان
Makoto Ito, Yu Yamamoto, Chul-Sa Kim, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba,