کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10881823 1077924 2005 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of fluid shear stress on apoptosis of cultured human umbilical vein endothelial cells induced by LPS
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوفیزیک
پیش نمایش صفحه اول مقاله
Effects of fluid shear stress on apoptosis of cultured human umbilical vein endothelial cells induced by LPS
چکیده انگلیسی
The objective of our research was to reveal the effects of shear stress on the apoptosis of cultured human umbilical vein endothelial cells (HUVECs) induced by lipopolysaccharide (LPS). A parallel-plate flow chamber was used to control the strength and duration of shear stress (SS), and apoptosis was measured by immunocytochemistry and radio-immunoassay. Some important conclusions were drawn. In the stationary state, apoptosis of HUVECs could be induced by LPS (50 μg/ml). An SS of 15 dyn/cm2 could inhibit the apoptosis induced by LPS. However, an SS of 4 dyn/cm2 had less effect on the same process. At the same time, the experiment demonstrated that the increase in IL-6 secretion by LPS can be inhibited by two different levels of shear stress. Moreover, the inhibition effect was more obvious under high level stress than under low level. We also found that the effect of shear stress on IL-8 was less effective than on IL-6. This research provides data for understanding the mechanism of the contribution of hemodynamic forces to atherosclerosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 29, Issue 11, November 2005, Pages 932-935
نویسندگان
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