کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10881862 | 1077927 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NMR measurements of the diffusional permeability of water in cultured colonic epithelial cancer cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
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چکیده انگلیسی
The water residence time and diffusional water permeability in colonic epithelial T84 cancer cells was measured using 1H NMR spectroscopy; the values estimated were 35.2 ± 2.8 ms and (7.4 ± 0.6) Ã 10â3 cm sâ1, respectively. Water permeability was inhibited to â¼10% of its original value by the mercurial diuretic, p-chloromercuribenzenesulfonate (PCMBS; 1 mM), and fully restored by dithiothreitol (DTT; 1 mM). The permeability was also inhibited reversibly to â¼55%, by extracellular glibenclamide (1 mM), an inhibitor of some ATP-binding cassette (ABC) transporters, including the cystic fibrosis transmembrane conductance regulator (CFTR). Addition of the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IMBX; 0.1-1 mM) and the adenylate cyclase activator, forskolin (0.1-1 mM) did not alter water permeability. It is concluded that in T84 cells water diffuses through the membrane lipid bilayer and via channels that are inhibited by PCMBS, including the channels that are known to be inhibited by glibenclamide.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 29, Issue 6, June 2005, Pages 441-448
Journal: Cell Biology International - Volume 29, Issue 6, June 2005, Pages 441-448
نویسندگان
M.H. Nouri-Sorkhabi, B.E. Chapman, E.V. O'Loughlin, Z. Li, P.W. Kuchel, K.J. Gaskin,