Mitochondrial SIRT4-type proteins in Caenorhabditis elegans and mammals interact with pyruvate carboxylase and other acetylated biotin-dependent carboxylases

► C. elegans SIRT4 orthologs function during oxidative stress in mitochondria. ► Conserved interaction of SIRT4 with mitochondrial biotin‐dependent carboxylases. ► All three carboxylases are acetylated on multiple lysine residues. ► Acetylation of K748 changed the enzymatic activity of pyruvate carboxylase (PC). ► No deacetylation or regulation of PC by SIRT4 in liver and HEK293 cells was found.