کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10883089 | 1078350 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of X-irradiation on mitochondrial DNA damage and its supercoiling formation change
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
OERRBEDSBsNHEJBERMitochondrial DNA - DNA میتوکندریاNER - DOWNLong PCR - PCR طولانیROS - ROSSSBs - SSBLET - اجازه دهیدIrradiation - اشعه یا تابشnucleotide excision repair - تعمیر مجدد نوکلئوتیدیbase excision repair - تعمیر پایه پایهcommon deletion - حذف معمولmtDNA - دیانای میتوکندریاییsingle strand breaks - شکاف تک رشته ایdouble strand breaks - شکست دو رشتهnon-homologous end joining - عدم پیوستن انتهای غیر همولوگReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of X-irradiation on mitochondrial DNA damage and its supercoiling formation change Effects of X-irradiation on mitochondrial DNA damage and its supercoiling formation change](/preview/png/10883089.png)
چکیده انگلیسی
There have been a small number of reports of radiation-induced mtDNA damage, and mtDNA supercoiling formation change induced by ionizing radiation has not been investigated before. This study evaluated mtDNA damage and supercoiling formation change after X-irradiation. The human breast cancer cell line, MCF-7 cells were used for analysis. Modified supercoiling-sensitive real-time PCR approach was used to evaluate mitochondrial DNA supercoiling formation change and copy number; long-PCR method was applied for the quantification of mtDNA damage. MtDNA damage and formation change induced by high-dose irradiation was persistent in 24Â h after irradiation and was not significant after low-dose irradiation. MtDNA copy number was slightly increased after high-dose irradiation and a transit increase was observed after low-dose irradiation. This is the first study to evaluate radiation-induced mitochondrial DNA supercoiling formation change using real-time PCR. Combined with data of ROS generation and dynamics of mitochondrial mass, our findings suggested that mtDNA is sensitive to radiation hazards, indicating mitochondrial biogenesis play an important role in radiation-induced cellular response.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mitochondrion - Volume 11, Issue 6, November 2011, Pages 886-892
Journal: Mitochondrion - Volume 11, Issue 6, November 2011, Pages 886-892
نویسندگان
Xin Zhou, Ning Li, Yanling Wang, Yipei Wang, Xin Zhang, Hong Zhang,