کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10883660 | 1078503 | 2005 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The use of whole genome amplification in the study of human disease
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کلمات کلیدی
SDAPEPFFPE, formalin-fixed paraffin embeddedDOP-PCRCGH, comparative genomic hybridization - CGH، هیبریداسیون ژنومی مقایسه ایLCM, Laser capture microdissection - LCM، microdissection ضبط لیزرLOH, loss of heterozygosity - LOH، از دست دادن هتروزیگوتیسمRFLP, restriction fragment length polymorphism - RFLP، polymorphism طول قطعه محدودیتwhole genome amplification - تقویت کل ژنومPCR, polymerase chain reaction - واکنش زنجیرهٔ پلیمرازSNP, single nucleotide polymorphism - چندریختی تک-نوکلئوتید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
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چکیده انگلیسی
The availability of large amounts of genomic DNA is of critical importance for many of the molecular biology assays used in the analysis of human disease. However, since the amount of patient tissue available is often limited and as particular foci of interest may consist of only a few hundred cells, the yield of DNA is often insufficient for extensive analysis. To address this problem, several whole genome amplification (WGA) methodologies have been developed. Initial WGA approaches were based on the polymerase chain reaction (PCR). However, recent reports have described the use of non-PCR-based linear amplification protocols for WGA. Using these methods, it is possible to generate microgram quantities of DNA starting with as little as 1Â mg of genomic DNA. This review will provide an overview of WGA approaches and summarize some of the uses for amplified DNA in various high-throughput genetic applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Progress in Biophysics and Molecular Biology - Volume 88, Issue 1, May 2005, Pages 173-189
Journal: Progress in Biophysics and Molecular Biology - Volume 88, Issue 1, May 2005, Pages 173-189
نویسندگان
Simon Hughes, Nona Arneson, Susan Done, Jeremy Squire,