کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10889326 1080826 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescence linked immunosorbant assays using microtiter plates
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Fluorescence linked immunosorbant assays using microtiter plates
چکیده انگلیسی
Fluorescence methods are widely used in the detection of antibodies and other binding events. However, as a general screening and detection tool in microtiter plates, enzyme linked immunosorbant (ELISA) methods predominate. In this paper we explore all parameters for effective use of fluorescence as a plate based detection method, including which microtiter plates can be used, the most effective means of immobilization, and the use of different fluorescent dyes or fluorescent proteins. These studies indicate that fluorescent immunosorbant assays (FLISA) can be used as effectively as enzymatic method in microtiter plate based screening methods, including the screening of phage antibody selections.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 336, Issue 2, 31 July 2008, Pages 135-141
نویسندگان
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